A gene‐cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production
- 1 January 1988
- journal article
- review article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 28 (4) , 211-220
- https://doi.org/10.1002/jobm.3620280402
Abstract
A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 μ/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1‐based DNA bank of K. lactis, the KEX1 gene involved in the killer system was isolated by complementation.Keywords
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