Measurement of anti‐thyroglobulin IgG in urine of patients with autoimmune thyroid diseases by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay)

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Abstract
Anti‐thyroglobulin IgG in urine of patients with Graves' disease and chronic thyroiditis and healthy subjects was measured by a sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay). Anti‐thyroglobulin IgG in dialyzed urine was reacted simultaneously with 2,4‐dinitrophenylated thyroglobulin and thyroglobulin‐βT‐D‐galactosidase conjugate. The immune complex formed consisting of the three components was trapped onto polystyrene balls coated with (anti‐2,4, dinitrophenyl group) IgG, eluted with ∈N‐2,4‐dinitrophenyl‐L‐lysine, and transferred onto polystyrene balls coated with (antihuman IgG γ‐chain) IgG. β‐D‐Galactosidase activity bound to the last polystyrene balls was assayed by fluorometry. Anti‐thyroglobulin IgG was detected in most of the patients, but not in most of the healthy subjects; levels of anti‐thyroglobulin IgG in urine of the patients were well correlated to those in serum of the same patients. The measurement of anti‐thyroglobulin IgG in urine by the immune complex transfer enzyme immunoassay was suggested to be useful as a diagnostic aid for autoimmune thyroid diseases. The conventional standard ELISA was not sufficiently sensitive for measuring anti‐thyroglobulin IgG in urine.

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