The Effect of Elution on the Reactivity of Antibodies of the ABO System, Including Cross‐reacting Antibodies, as Demonstrated by Use of Red Cells of Various Subgroups of A, and Group B

Publisher Website
Google Scholar
Abstract
Summary. The combining capacity of various A antigens with the corresponding antibodies was investigated. A modified Landsteiner elution technique was the principal method used and eluates were made at various temperatures ranging from 22 to 65° C. The ability of the eluates to agglutinate Ax cells was inversely proportional to the amount of A activity possessed by the cells used for elution. For example, titration values against Ax cells of eluates from A1, A2, A3, Am and Ax cells were 0, 4, 8, 16 and 32 with each type of cell respectively. The highest titre (128) against Ax cells was found in an eluate from B cells, this reaction being entirely due to cross‐reacting antibodies, i.e. antibodies agglutinating both A and B cells, present in the eluate. The results suggest differences in the nature of the combination between anti‐A and the A antigen present in the various A subgroups, and in the combining capacity of the cross‐reacting antibody with cells of group A compared with B. The possibility that alteration of the configuration of the antibody molecules through elution may be responsible for some of the observed results, is discussed.