Improved transfer of high molecular weight proteins using fabric‐reinforced soft polyacrylamide gels
- 1 January 1988
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 9 (5) , 234-236
- https://doi.org/10.1002/elps.1150090509
Abstract
Rapid transfer of electrophoretically separated, high molecular weight proteins from fabric‐reinforced, soft, low‐concentration polyacrylamide gels (3.5 %T, 2.6 %C) is described. Polyester fabrics proved useful for gel reinforcement during vertical slab gel electrophoresis. The fabric‐reinforced gel retains its dimension during transfer, equilibration and drying, facilitating comparison of protein bands in the separation gel and transfer matrix.This publication has 15 references indexed in Scilit:
- Anchoring and Biosynthesis of Stalked Brush Border Membrane Proteins: Glycosidases and Peptidases of Enterocytes and Renal TubuliAnnual Review of Cell Biology, 1986
- Rapid electrotransfer of proteins from polyacrylamide gel to nitrocellulose membrane using surface-conductive glass as anodeAnalytical Biochemistry, 1985
- Protein blotting in uniform or gradient electric fieldsAnalytical Biochemistry, 1985
- Fabric reinforced polyacrylamide gels for electroblottingElectrophoresis, 1985
- Blotting intact immunoglobulins and other high-molecular-weight proteins after composite agarose-polyacrylamide gel electrophoresisAnalytical Biochemistry, 1984
- India ink staining of proteins on nitrocellulose paperAnalytical Biochemistry, 1983
- Protease-facilitated transfer of high-molecular-weight proteins during electrotransfer to nitrocelluloseAnalytical Biochemistry, 1981
- Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid ProteinsScience, 1981
- Immunoelectrophoretic studies on pig intestinal brush border proteinsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1977