Holin locus characterisation from lysogenic Xenorhabdus nematophila and its involvement in Escherichia coli SheA haemolytic phenotype

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Abstract
Lysogeny has previously been described in the entomopathogenic bacteria of the genus Xenorhabdus. Screening of a X. nematophila prophage DNA library on blood agar resulted in the identification of a 5.7-kb locus that caused a haemolytic phenotype when cloned in Escherichia coli, but not in the E. coli sheA null mutant, lacking the SheA cryptic haemolysin. This locus exhibited similarity to lysis genes from lambdoid phages. In particular, it encoded a functional holin able to complement a lambda Sam7 mutant. It is the second time that a locus encoding a functional holin is shown to reveal the SheA haemolytic phenotype in E. coli. The possible role of the holin in extracellular release of SheA is discussed

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