Isolation and Culture of Human Decidual Capillary Endothelial Cells in Serum-Free Medium Supplemented with Human Uterine Angiogenic Factor

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Abstract
Human decidual capillary endothelial (HDCE) cells obtained from decidual fragments of legally induced first-trimester terminations of pregnancies, were cultured in a serum-free medium supplemented with human uterine angiogenic factor (HUAF). The method of isolating the cells from the decidual tissue is described. Identification of the decidual endothelial cells was based on light- and electron-microscopic observations as well as on antifactor VIII immu-noperoxidase-staining technique. The HDCE cell culture in the serum-free medium lasted for 25 weeks through eight subcultures. The cells frozen in glycerin yielded 80% viability after thawing. Electron-microscopic observations of the monolayer demonstrated Weibel-Palade bodies desmosomes and tight junctions. HUAF is mitogenic to HDCE at 10–100 ng/ml. The dependency of HDCE cells grown in culture on HUAF may explain in part the mechnism involved in the dynamic vascular expansion occurring during gestation.

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