Applications of Lc/Ms in Forensic Chemistry

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Abstract
Various analytical techniques for highly sensitive liquid chromatography / mass spectrometry (LCIMS) and their applications in forensic chemistry have been investigated The following three types of LCMS instruments were used for this study 1) a Micromass model PLATFORM II equipped with an atmospheric pressure chemical ionization (APCI) or an electrospray ionization (ESI) interface, 2) a Shimadzu model QP-1100EX combined with a thermospray TSP interface; 3) a JEOL model JMS-SX102A fitted with a Frit-fast atom bombardment ionization (Frit-FAB) interface Optimization of capillary voltage and the flon rate of the inobile phase were found for ESI-LCfilS The relationship between the composition of mobile phase and ionization efficiency was investigated in the ESI, APCI and TSP types of LC/MS In ESI-LC/MS analysis. the use of a semi-micro column (15 mm 1D) allowed about ten times more sensitive an analysis than a conventional column (46 mm ID) In order to examine the provable period of triazolam, which is quickly metabolized, urine samples obtained from four volunteers who had taken 0.25 or 0 5 mg of the drug were analyzed by TSP-LCIMS The main urinary metabolite of triazolam, α -hydroxytriazolam (α -HT) was detectable up to 36 hours after taking the dosage. For the purpose of checking the detectable period of suxamethonium in post-mortem biological samples, Frit-FAE3 LC/MS analysis was performed for the tissue samples of the rats which died by the intraperitoneal administration of the drug at 10 mg/kg bodyweight. Suxamethonium in the rat liver was detectable up to 42 days later, as long as the bodies were stored at 0°C immediately after the death

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