Cytological characterisation of heterochromatin in mitotic and meiotic chromosomes of the Old World screwworm fly, Chrysomya bezziana (Diptera: Calliphoridae)

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Abstract
Mitotic and meiotic chromosomes of the Old World screwworm fly, Chrysomya bezziana, were studied using C-banding and quinacrine and counterstain-enhanced fluorescence techniques. The five autosomes in the karyotype are evenly graded in size, with somewhat variable arm ratios. Distinguishing all autosomes on these features alone can be difficult. C-banding produces small centromeric bands in the autosomes, whereas the much longer X and Y chromosomes have extensive dark C-band blocks with intermediate background staining. Most bright fluorescence occurs in the sex chromosomes, particularly the X chromosome, which has remarkable banding detail. Band resolution is greatly increased in mitotic metaphase cells from embryos. Quinacrine staining of mitotic chromosomes produces bright fluorescence at the centromere regions of chromosomes 2, 3, and 4, assisting in their identification. Meiotic chromosomes have distinctly reduced brightness and resolution of fluorescent bands and show marked chromatid asynapsis in the brighter regions of the sex chromosomes. Fluorochromes staining A∙T-rich DNA (quanacrine and 4,6-diamidino-2-phenylindole (DAPI)) produce bright staining in a large proportion of the sex chromosomes. By contrast chromomycin, which binds preferentially to G∙C-rich DNA, stains a much smaller proportion of the sex chromosomes than expected from reciprocal staining. Together with the asynapsis data this indicates that much of the heterochromatin in the sex chromosomes has unusual structural properties.Key words: Chrysomya bezziana, screwworm, karyotype, C-banding, fluorescence, heterochromatin.

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