Angiotensin II–Induced Phosphorylation of the AT1Receptor From Rat Brain Neurons
- 1 September 1997
- journal article
- research article
- Published by Wolters Kluwer Health in Hypertension
- Vol. 30 (3) , 351-357
- https://doi.org/10.1161/01.hyp.30.3.351
Abstract
The neuronal angiotensin II (Ang II) type 1 (AT1) receptor is coupled to the Ras-Raf-1–mitogen-activated protein (MAP) kinase signal-transduction pathway (Yang H, Lu D, Yu K, Raizada MK. Regulation of neuromodulatory actions of angiotensin II in the brain neurons by the Ras-dependent mitogen-activated protein kinase pathway. J Neurosci. 1996;16:4047-4058). In this study we compared the effects of angiotensin II (Ang II) on AT1 receptor phosphorylation and the ability of the phosphorylated receptor to bind Ang II in neuronal cultures of Wistar-Kyoto rat (WKY) and spontaneously hypertensive rat (SHR) brains to further our understanding of the Ang II signaling mechanism. Ang II caused a time-dependent phosphorylation of AT1 receptors in both WKY and SHR brain neurons. The level of phosphorylation was higher in the SHR brain neurons; this finding was consistent with increased AT1 receptors in these cells. MAP kinase was involved in this phosphorylation, a conclusion supported by the following evidenc...Keywords
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