A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay.
Open Access
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 7 (1) , 549-551
- https://doi.org/10.1128/mcb.7.1.549
Abstract
The mechanism by which adenovirus virus-associated RNAI stimulates translational efficiency in a transient-expression assay in 293 cells was investigated. We showed that DNA transfection leads to activation of a protein kinase that phosphorylates the alpha subunit of eucaryotic initiation factor 2 and, as a consequence, inhibition of polypeptide chain initiation. Cotransfection of a plasmid encoding adenovirus type 2 virus-associated RNAI recovered the translational capacity by preventing activation of the kinase.This publication has 25 references indexed in Scilit:
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