Effect of direct current on cultured growth cartilage cells in vitro

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Abstract
Cultured growth cartilage cells of young rabbits were stimulated by three levels(10, l and 0.l μA) of constant direct current (DC). The effect of DC was examined by measuring the activities of proteoglycan synthesis (uptake of [35S]sodium sulfate and toluidine blue staining) and DNA synthesis (uptake of [3H]thymidine). DC increased syntheses of both proteoglycans and DNA when l μA calculated curren densiy of l μA/cm2 was applied for 3 days. A longer stimulation resulted in a larger difference between stimulated and unstimulted cells. Ten-microampere stimulation devitalized the cells, while 0.l μA had no effect on cell activities.