New Media for Rapid Growth ofSpiroplasma citriand Corn Stunt Spiroplasma

1 January 1984

journal article
research article
Published by Scientific Societies in Phytopathology®

Vol. 74 (1) , 84-89
https://doi.org/10.1094/phyto-74-84

Abstract

Several media (LD series) for cultivation of S. citri and corn stunt (CS) spiroplasma were developed by study of growth-promoting factors and eventual reformulation of a complex medium (L) originally developed for cultivation of S. citri. The maximum titer of S. citri grown in one of the new media, LD8, reached over 6 .times. 109 colony-forming units (CFU)/ml, with an estimated doubling time of about 4 h at 31.degree. C. In another medium, LD8A, CS spiroplasma (strain I-747) grew to a titer of about 2 .times. 109 CFU/ml, with a doubling time of 11-12 h at 31.degree. C. Media LD8 and LD8A were highly suitable for primary isolation of S. citri and CS spiroplasma, respectively, from diseased plants [Zea mays and Catharanthus roseus].

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