Delivery of Short Hairpin RNA Sequences by Using a Replication-Competent Avian Retroviral Vector
Open Access
- 1 May 2004
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 78 (9) , 4914-4916
- https://doi.org/10.1128/jvi.78.9.4914-4916.2004
Abstract
While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.Keywords
This publication has 25 references indexed in Scilit:
- Lentivirus-delivered stable gene silencing by RNAi in primary cellsRNA, 2003
- A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interferenceNature Genetics, 2003
- An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivoNature Genetics, 2003
- Stable suppression of tumorigenicity by virus-mediated RNA interferenceCancer Cell, 2002
- Induction of ovarian cancer by defined multiple genetic changes in a mouse model systemCancer Cell, 2002
- Development of an Avian Leukosis-Sarcoma Virus Subgroup A Pseudotyped Lentiviral VectorJournal of Virology, 2001
- Development of a flexible and specific gene delivery system for production of murine tumor modelsOncogene, 1999
- Targeted Gene Misexpression in Chick Limb Buds Using Avian Replication-Competent RetrovirusesMethods, 1998
- A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptorCell, 1993