Cellular localization of the receptor‐dependent and receptor‐independent uptake of human LDL in the liver of normal and 17α‐ethinyl estradiol‐treated rats
- 5 April 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 154 (1) , 75-80
- https://doi.org/10.1016/0014-5793(83)80878-3
Abstract
The cellular localization in the liver of the receptor-dependent and -independent uptake of human low density lipoprotein (LDL) in normal and 17α-ethinyl estradiol-treated rats was investigated by the simultaneous in vivo injection of human 131I-LDL and human reductive methylated 125I-LDL. The cells were subsequently isolated by a low temperature method. In untreated rats, after 30 min of in vivo circulation of human LDL, 57% of the receptor-dependent liver-association of human LDL occurs in non-parenchmal cells and 43% in parenchymal cells. Estradiol treatment of rats for 3 days selectively increases the receptor-dependent cell-association of human LDL with hepatocytes (17-fold), while the receptor-dependent cell-association with non-parenchymal cells is not affected.Keywords
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