Demonstration of N -acetylchondrosine-degrading β-glucuronidase in rabbit liver
- 4 July 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 234 (1) , 91-94
- https://doi.org/10.1016/0014-5793(88)81310-3
Abstract
N-Acetylchondrosine was incubated at pH 4.0 with a rabbit-liver crude enzyme extract. Gel filtration of the reaction products on Sephadex G-15 revealed the presence of monosaccharide liberated from the disaccharide. The monosaccharide fraction was analyzed by gas-liquid chromatography, and identified as a mixture of glucuronic acid and N-acetylgalactosamine. These results indicate the presence of β-glucuronidase, which degrades N-acetylchondrosine, in rabbit liver. The discovery of the presence of this enzyme may help to establish the complete degradation process of chondroitin sulfatesKeywords
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