A MICROMETHOD FOR MEASUREMENT OF RENIN IN THE PLASMA AND KIDNEY OF RATS
- 1 September 1967
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Physiology and Pharmacology
- Vol. 45 (5) , 881-890
- https://doi.org/10.1139/y67-103
Abstract
A new micromethod for the measurement of renin activity and substrate in 0.1 of rat plasma and 1 mg of rat renal tissue is described. A rat substrate free of renin prepared from previously nephrectomized animals is used. Incubation is carried out in the presence of Dowex resin 50W-X2 (NH4+) for 12 hours, at pH 6.5, with an excess of rat substrate. Angiotensin formed during incubation is measured by a pressor assay in the rat. The reprodu-cibility of the procedure was excellent. Recovery of angiotensin in amounts from 10 to 500 mug was 77%. This method permits repeated measurements of renin activity in rats during acute experiments and in any physiological procedure. It can readily be adapted to other species.This publication has 3 references indexed in Scilit:
- A METHOD FOR ESTIMATION OF RENIN ACTIVITY IN PLASMA1965
- The Quantitative Determination of Renin in the Plasma of Patients with Arterial HypertensionCirculation, 1963
- Assay of Renin in Peripheral BloodThe Tohoku Journal of Experimental Medicine, 1963