Temperature Dependence of Ionizing Radiation Effect on Dry Hyaluronidase

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Abstract
Dry bovine testicular hyaluronidase in relatively impure preparation has been irradiated with fast electrons at temperatures from 66[degree]K to 433[degree]K. On subsequent recovery of the hyaluronidase into solutions it was assayed for enzymatic activity in digestion of hyaluronic acid to yield detectable oligosaccharides. The activity remaining was found to be approximately logarithmic in relation to the electron dose given. With correction for recovered protein the results as a function of temperature can be reasonably fitted by the sum of two terms, one independent of temperature, and the other characterized by an Arrhenius activation energy of about 1100 cal/mole. In relation to similar work with other enzymes, a larger activation energy usually found at higher temperatures is negligible for hyaluronidase.

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