Ultrafiltration as a Method for Concentrating Rift Valley Fever Virus Grown in Tissue Culture
Open Access
- 1 January 1971
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 21 (4) , 758-760
- https://doi.org/10.1128/aem.21.4.758-760.1971
Abstract
Filtration by means of a Diaflo ultrafilter was used to concentrate three 1,000-ml lots and one 3,000-ml lot of tissue culture-grown Rift Valley fever virus. Quantitation of both infectivity and total protein was achieved. Water treatment with continued ultrafiltration of the virus concentrate provided a final virus product approximately 99.25% free of low-molecular-weight materials originally present in the growth medium.Keywords
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