Probability of Normal Development after Transplantation of Fertilized Rabbit Ova Stored at Different Temperatures

Publisher Website
Google Scholar
Abstract
The detailed results of transplantations of rabbit ova recovered at different stages and stored at different temps. for various length of time were reported. About 54, 31, or 23% of freshly recovered 2-celled ova, morulae or blastocysts were developed into normal young. About 25% of development was obtained when 2-celled ova were cultured at 38 C for 2 days. About 37, 36 or 18% of development was obtained, respectively, when the 2-celled ova, morulae, or blastocysts were stored at 10 C for 24 hrs. Ova at 2-cell stage or at morula can be stored at 10 C for 4 days but the % of development was rather low. Very low % of development was obtained after the 2-celled ova were stored at 0 C for 1-4 days. No development was observed when 2-celled ova were transplanted into uteri. The % of ova failing to implant was high when the ova were recovered at a late stage and/ or when they were stored. The % of degeneration was high after longer time of storage. The defective ova or embryos were degenerated rather than developed into abnormal young. There was no obvious differential mortality of male or female zygotes treated at different temps. Large number of ova produced by gonadotrophin administration were capable of developing into normal young.

This publication has 2 references indexed in Scilit: