The enhancers and promoters of theXenopus laevis; ribosomal spacer are associated with histones upon active transcription of the ribosomal genes
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (21) , 6393-6397
- https://doi.org/10.1093/nar/18.21.6393
Abstract
The presence of histones on the enhancer-promoter region of the X. laevis ribosomal spacer has been studied in embryos at stage 40, where the ribosomal genes are actively transcribed. Isolated tadpole nuclei were either fixed with formaldehyde or irradiated with UV laser to crosslink histones to DNA. The purified protein-DNA complexes were immunoprecipitated with antibodies to the histones H1, H2A and H4 and the DNA fragments carrying the respective histones were analyzed for the presnece of spacer enhancer-promoter sequences by hybridization to specific DNA probe. The two independent crosslinking procedures revealed the presence of these DNA sequences in the precipitated DNA. The quantitative analysis of the UV laser-crosslinked complexes showed that histones H2A and H4 were associated with enhancer-promoter DNA in amounts similar to those found for bulk DNa, whilst the content of H1 was reduced.This publication has 35 references indexed in Scilit:
- Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonucleasePublished by Elsevier ,2004
- Interactions of acetylated histones with DNA as revealed by UV laser induced histone-DNA crosslinkingBiochemical and Biophysical Research Communications, 1989
- Sites of topoisomerase I action on X. laevis ribosomal chromatin: Transcriptionally active rDNA has an ∼200 bp repeating structureCell, 1988
- Accessibility of the globular domain of histones H1 and H5 to antibodies upon folding of chromatinEuropean Journal of Biochemistry, 1987
- A complex array of sequences enhances ribosomal transcription in Xenopus laevisJournal of Molecular Biology, 1987
- Structure of the extrachromosomal ribosomal RNA chromatin of Physarum polycephalumJournal of Molecular Biology, 1987
- Chromatin structure of hsp 70 genes, activated by heat shock: Selective removal of histones from the coding region and their absence from the 5′ regionCell, 1984
- Reversible changes in nucleosome structure and histone H3 accessibility in transcriptionally active and inactive states of rDNA chromatinCell, 1983
- Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoterCell, 1982
- Nucleosome structure of Xenopus oocyte amplified ribosomal genesBiochemistry, 1978