Leucocyte‐Associated Plasma Proteins

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Abstract
Sixteen plasma proteins were studied in the supernatants from 20 successive washings of human leucocytes by a new sensitive immunoelectropharetic method. In the first few supernatants u fast and parallel decrease in concentration of all proteins look place, but for 11 proteins this was followed by a phase of slow decrease due to cellular release. Eight proteins could be quantitated throughout the 20 washings: prealbumin, albumin, orosomucoid, α1‐antitrypsin, transferrin, C3, haptoglobin, and IgG. These proteins and trace amounts of eight other plasma proteins were present in the cell lysate after the washings. In comparison with plasma, the leucocytes contained relatively higher concentrations of prealbumin, orosomucoid, α1‐antitrypsin, and haptoglobin than of albumin. In crossed immunoelcctrophoresis these same four proteins were present as molecular variants differing from their plasma counterpart in elcetrophoretic mobility and morphology of the precipitate.

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