Identification of the structural proteins of an ATP-driven potassium transport system in Escherichia coli.

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Abstract
The 3 structural proteins of the ATP-driven Kdp K transport system of E. coli were identified and were located in the inner membrane. The high-affinity repressible Kdp system is 1 of 4 K transport systems in E. coli. The Kdp proteins were identified both in growing cells and in heavily UV-irradiated cells infected with transducing phages carrying the kdp operon. Although all previously identified ATP-driven transport systems of gram-negative bacteria contained a periplasmic protein component, no evidence was found for such a component or for an outer membrane component of the Kdp system. The MW of the 3 inner membrane proteins, KdpA, KdpB and KdpC, were 47,000, 90,000 and 22,000, respectively.