Cyclic AMP in sheep ovarian follicles: site of production and response to gonadotrophins

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Abstract
This study was undertaken to establish a relationship between cyclic (c) AMP production and the degree of LH [luteinizing hormone] and FSH [follicle stimulating hormone] stimulation; to determine the effects of various gonadotropins on follicular formation of cAMP; and to identify the precise intrafollicular site of cAMP formation. The formation of cAMP increased rapidly in follicles exposed to LH. Maximum concentrations were reached after 90 min and were maintained for 180 min. Extracellular release of cAMP increased steadily throughout the 180-min experimental period. Tissue levels of cAMP increased proportionally and significantly when LH concentrations in the medium were increased from 0 to 200 mIU ml-1. Tissue levels of cAMP were significantly increased by human chorionic gonadotropin, prostaglandin (PG) E2 and noradrenaline, but not by prolactin, PGF2.alpha., serotonin or melatonin. cAMP formation occurred predominantly in the thecal compartment; the membrana granulosa contributed less than 3% of the total amount of cAMP formed after gonadotrophic stimulation. A significant amount of cAMP from the thecal cells was released into the extracellular compartment and appeared to pass into the granulosa cells.
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