The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X
- 15 February 2001
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 409 (6822) , 942-943
- https://doi.org/10.1038/35057165
Abstract
We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.Keywords
This publication has 11 references indexed in Scilit:
- Integration of telomere sequences with the draft human genome sequenceNature, 2001
- A physical map of human chromosome 14Nature, 2001
- A high-resolution map of human chromosome 12Nature, 2001
- A physical map of the human Y chromosomeNature, 2001
- A physical map of the human genomeNature, 2001
- Initial sequencing and analysis of the human genomeNature, 2001
- The DNA sequence of human chromosome 21Nature, 2000
- The DNA sequence of human chromosome 22Nature, 1999
- A 9.75-Mb Map across the Centromere of Human Chromosome 10Genomics, 1996
- Parameters of the human genome.Proceedings of the National Academy of Sciences, 1991