Mechanism of Activation by Adenosine 3′:5′-Cyclic Monophosphate of a Protein Phosphokinase from Rabbit Reticulocytes
- 1 September 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (1) , 408-414
- https://doi.org/10.1073/pnas.67.1.408
Abstract
Two protein phosphokinases (EC 2.7.1.37) were found to be present in rabbit reticulocytes. The two enzymes were separated by DEAE-cellulose chromatography and called kinases I and II. Adenosien 3':5'-cyclic monophosphate stimulated the activity of both enzymes. However, the degree of stimulation was different and depended on the protein acceptor used. In the presence of adenosine 3':5'-cyclic monophosphate, protein kinase I dissociated into two subunits: a subunit binding adenosine 3':5'-cyclic monophosphate, and a catalytic subunit. The component binding the cyclic nucleotide appeared to act as an inhibitory protein, regulating the activity of the catalytic subunit. The mechanism of action of the cyclic nucleotide on kinase II appeared to be different from that of kinase I.Keywords
This publication has 11 references indexed in Scilit:
- A cyclic-3′,5′-adenosine monophosphate dependent protein kinase from the adrenal cortex: Comparison with a cyclic AMP binding proteinBiochemical and Biophysical Research Communications, 1970
- Cyclic nucleotide-dependent protein kinases. 3. Purification and properties of adenosine 3',5'-monophosphate-dependent protein kinase from bovine brain.1969
- CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASES, IV. WIDESPREAD OCCURRENCE OF ADENOSINE 3′,5′-MONOPHOSPHATE-DEPENDENT PROTEIN KINASE IN VARIOUS TISSUES AND PHYLA OF THE ANIMAL KINGDOMProceedings of the National Academy of Sciences, 1969
- A STIMULATION BY CYCLIC 3′,5′-ADENOSINE MONOPHOSPHATE OF AMINO ACID ACTIVATION AND POLYMERIZATION IN RETICULOCYTE HEMOLYSATESProceedings of the National Academy of Sciences, 1969
- A cyclic AMP - stimulated protein kinase in adipose tissueBiochemical and Biophysical Research Communications, 1969
- ISOLATION OF ADENYL CYCLASE FROM Escherichia coliProceedings of the National Academy of Sciences, 1969
- Histone Phosphorylation: Stimulation by Adenosine 3′,5′-MonophosphateScience, 1968
- An Adenosine 3′,5′-Monophosphate-dependant Protein Kinase from Rabbit Skeletal MuscleJournal of Biological Chemistry, 1968
- A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein MixturesJournal of Biological Chemistry, 1961
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951