First Preparation of Biotinylated Gradient Polyethylene Surface to Bind Photoactive Caged Streptavidin
- 22 March 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 21 (9) , 4066-4070
- https://doi.org/10.1021/la046868a
Abstract
A gradient polyethylene (PE) surface was created through corona treatment, in which the corona power increased along the 5 cm length of the PE. The gradient surface was treated with polyethyleneimine and then biotin. Fluorescein-conjugated streptavidin (SAV) caged within 5-carboxymethoxy-2-nitrobenzyl (CMNB) adsorbed onto the biotinylated gradient PE surface following molecular recognition principles. Photoirradiation decomposed the CMNB cage and allowed the fluorescein-conjugated SAV to fluoresce, the intensity of which increased gradually along the PE surface.Keywords
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