ANTIGEN-SPECIFIC T-CELL-MEDIATED SUPPRESSION .2. IN VITRO INDUCTION BY I-J-CODED L-GLUTAMIC ACID-50 -L-TYROSINE-50 (GT)-SPECIFIC T-CELL SUPPRESSOR FACTOR (GT-TSF) OF SUPPRESSOR T-CELLS (TS2) BEARING DISTINCT I-J DETERMINANTS

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 121  (2) , 602-607
Abstract
The induction of new suppressor T [thymus derived] cells (Ts2) by suppressive extracts (TsF) from L-glutamic acid50-L-tyrosine50 (GT) nonresponder mice was examined. Incubation of normal spleen cells with allogeneic GT-TsF for 2 days in vitro led to the generation of Ts2 cells able to suppress subsequent responses to the immunogen GT-methylated bovine serum albumin (GT-MBSA) in vivo. This induction occurred efficiently when TsF donor and target cells differed at all of H-2, including the I-J subregion. B10.BR (H-2k) GT-TsF, adsorbed on, then acid eluted from GT-Sepharose and anti-I-J [B10.A (3R) anti-B10.A (5R)]-Sepharose in a sequential fashion could induce BALB/c (H-2d) spleen cells to become Ts2 only if nanogram quantities of GT were added to the purified GT-TsF. This indicates a requirement for a molecule or molecular complex possessing I-J determinants and antigen (GT)-binding specificity, together with GT itself, for Ts2 induction. The induced Ts2 are I-J+, since their function can be eliminated by treatment with anti-I-Jk plus C [complement]. These I-J determinants are coded for by the precursor of the Ts2 and do not represent passively adsorbed, I-J coded TsF, since anti-I-Jk antiserum [(3R .times. DBA/2)F1 anti-5R] which cannot recognize the BALB/c (I-Jd) TsF used for induction still eliminates the activity of induced A/J (I-Jk) Ts2. These data provide further evidence for and information about the minimum of 2 T cells involved in antigen-specific suppressor T cell systems.

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