Cloning, mapping, and sequencing of the gene encoding Escherichia coli quinoprotein glucose dehydrogenase
- 1 November 1990
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 172 (11) , 6308-6315
- https://doi.org/10.1128/jb.172.11.6308-6315.1990
Abstract
Escherichia coli contains pyrroloquinoline quinone-dependent glucose dehydrogenase. We cloned and sequenced the gene (gcd) encoding this enzyme and showed that the derived amino acid sequence is highly homologous to that of the gdhA gene product of Acinetobacter calcoaceticus. Stretches of homology also exist between the amino acid sequence of E. coli glucose dehydrogenase and other pyrroloquinoline quinone-dependent dehydrogenases from several bacterial species. The position of gcd on the chromosomal map of E. coli was determined to be at 3.1 min. ImagesThis publication has 35 references indexed in Scilit:
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