Coregulation of Fibronectin Signaling and Matrix Contraction by Tenascin-C and Syndecan-4
Open Access
- 1 December 2004
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 15 (12) , 5670-5677
- https://doi.org/10.1091/mbc.e04-08-0759
Abstract
Syndecan-4 is a ubiquitously expressed heparan sulfate proteoglycan that modulates cell interactions with the extracellular matrix. It is transiently up-regulated during tissue repair by cells that mediate wound healing. Here, we report that syndecan-4 is essential for optimal fibroblast response to the three-dimensional fibrin-fibronectin provisional matrix that is deposited upon tissue injury. Interference with syndecan-4 function inhibits matrix contraction by preventing cell spreading, actin stress fiber formation, and activation of focal adhesion kinase and RhoA mediated-intracellular signaling pathways. Tenascin-C is an extracellular matrix protein that regulates cell response to fibronectin within the provisional matrix. Syndecan-4 is also required for tenascin-C action. Inhibition of syndecan-4 function suppresses tenascin-C activity and overexpression of syndecan-4 circumvents the effects of tenascin-C. In this way, tenascin-C and syndecan-4 work together to control fibroblast morphology and signaling and regulate events such as matrix contraction that are essential for efficient tissue repair.Keywords
This publication has 44 references indexed in Scilit:
- Tenascin-C Modulates Matrix Contraction via Focal Adhesion Kinase– and Rho-mediated Signaling PathwaysMolecular Biology of the Cell, 2002
- Regulatory Role for Src and Phosphatidylinositol 3-Kinase in Initiation of Fibronectin Matrix AssemblyJournal of Biological Chemistry, 2002
- Syndecan-4 as Antithrombin Receptor of Human NeutrophilsBiochemical and Biophysical Research Communications, 2001
- Identification of a novel heparin-binding site in the alternatively spliced IIICS region of fibronectin: roles of integrins and proteoglycans in cell adhesion to fibronectin splice variantsMatrix Biology, 2001
- Delayed wound repair and impaired angiogenesis in mice lacking syndecan-4Journal of Clinical Investigation, 2001
- Participation of Syndecan 2 in the Induction of Stress Fiber Formation in Cooperation with Integrin α5β1: Structural Characteristics of Heparan Sulfate Chains with Avidity to COOH-Terminal Heparin-Binding Domain of FibronectinExperimental Cell Research, 2000
- Requirements for α5β1Integrin-mediated Retraction of Fibronectin-Fibrin MatricesPublished by Elsevier ,1999
- ECM signalling: orchestrating cell behaviour and misbehaviourTrends in Cell Biology, 1998
- Syndecans-1 and -4 Are Induced During Wound Repair of Neonatal but Not Fetal SkinJournal of Investigative Dermatology, 1996
- The alternatively spliced V region contributes to the differential incorporation of plasma and cellular fibronectins into fibrin clots.The Journal of cell biology, 1992