Determination of placental estriol in urine by gas-liquid chromatography, with equilenin as internal standard.
Open Access
- 1 February 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (2) , 165-168
- https://doi.org/10.1093/clinchem/23.2.165
Abstract
Placental estriol in urine can be determined by rapid enzymatic hydrolysis, extraction with neutral XAD-2 resin, and quantitation by gas-liquid chromatography, with equilenin as the internal standard. Glucose, methenamine mandelate, hydrochlorothiazide, phenolphthalein, or salicylate do not interfere. Analytical recovery of estriol added to urine was 92-108%. Day-to-day precison (CV), established by 15 repilicate analyses at a mean concentration of 21.3 mg/liter, was 6.6%.This publication has 1 reference indexed in Scilit:
- β-GLUCURONIDASE HYDROLYSIS OF URINARY CORTICOSTEROID CONJUGATES: THE EFFECT OF SALICYLATE GLUCURONOSIDE AS A COMPETING SUBSTRATE AND THE EFFECT OF ENZYME INACTIVATION*Journal of Clinical Endocrinology & Metabolism, 1960