An Improved Method for Culturing Tradescantia Pollen Tubes for Chromosomal Analysis

1 January 1967

journal article
research article
Published by International Society of Cytology in CYTOLOGIA

Vol. 32 (1) , 1-5
https://doi.org/10.1508/cytologia.32.1

Abstract

Using pollen of Tradescantia paludosa, growth was greatly enhanced and mitosis accelerated in a medium containing Ca, Mg and K ions, at a high pH. The Ca-supplemented medium, unlike conventional pollen growth media, caused the mitotic chromosomes to align end to end in the pollen tubes. This improved distinctness, and led to more analyzable chromosomes in the pollen tubes.

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