The structure and function of a soybean β-glucan-elicitor-binding protein
- 4 February 1997
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 94 (3) , 1029-1034
- https://doi.org/10.1073/pnas.94.3.1029
Abstract
β-Glucan elicitor (GE), released from the cell wall of the phytopathogenic fungus Phytophthora megasperma by soybean glucanases, causes defense reactions in soybean. A GE-binding protein (GEBP) was purified from the membrane fraction of soybean root cells, and its cDNA was isolated. Expression of the cDNA clone in tobacco suspension cultured cells and in Escherichia coli conferred GE-binding activity to both. An antibody against the recombinant protein was found to inhibit the GE binding with the soybean cotyledon membrane fraction as well as the resulting accumulation of phytoalexin. Immunolocalization assays indicated that the GEBPs are located in the plasma membrane of root cells. These results suggest that the cDNA encodes a GE receptor and may mediate the signaling of the elicitor.Keywords
This publication has 42 references indexed in Scilit:
- One‐step purification of the β‐glucan elicitor‐binding protein from soybean (Glycine max L.) roots and characterization of an anti‐peptide antiserumFEBS Letters, 1996
- Plant transmembrane receptors: new pieces in the signaling puzzleTrends in Biochemical Sciences, 1996
- A Structural Model for the Mechanisms of Elicitor Release from Fungal Cell Walls by Plant [beta]-1,3-EndoglucanasePlant Physiology, 1995
- Solubilization of Functional Plasma Membrane-Localized Hepta-[beta]-Glucoside Elicitor-Binding Proteins from SoybeanPlant Physiology, 1993
- Identification of a novel high‐affinity binding site for N‐acetylchitooligosaccharide elicitor in the membrane fraction from suspension‐cultured rice cellsFEBS Letters, 1993
- S‐Carboxymethylation of proteins transferred onto polyvinylidene difluoride membranes followed by in situ protease digestion and amino acid microsequencingElectrophoresis, 1992
- Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus RNA using an improved electroporation methodFEBS Letters, 1987
- Release of a Soluble Phytoalexin Elicitor from Mycelial Walls of Phytophthora megasperma var. sojae by Soybean TissuesPlant Physiology, 1981
- Glyceollin: its rôle in restricting fungal growth in resistant soybean hypocotyls infected with Phytophthora megasperma var. sojaePhysiological Plant Pathology, 1978
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962