BETA-HEXOSAMINIDASE ISOZYMES IN HUMAN COLONIC CARCINOMA

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (3) , 731-735
Abstract
Isoelectric focusing of crude extracts demonstrated that human colonic carcinomas contained a higher proportion of .beta.-hexosaminidase B than .beta.-hexosaminidase A, while normal human colonic mucosa contained a higher proportion of the A form of the enzyme. Studies of the isolated isozymes showed that .beta.-hexosaminidase B was more stable to heat and more active at low pH than .beta.-hexosaminidase A. Kinetic studies revealed that A and B forms of .beta.-hexosaminidase had essentially the same Km and Vmax for p-nitrophenyl-N-acetyl-.beta.-D-glucosaminide.

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