The complete primary structure of the human snRNP E protein
- 25 November 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (22) , 10593-10605
- https://doi.org/10.1093/nar/16.22.10593
Abstract
The snRNP E protein is one of four "core" proteins associated with the snRNAs of the U family (U1, U2, U4, U5, and U6). Screening of a human teratoma cDNA library with a partial cDNA for a human autoimmune antigen resulted in the isolation of a cDNA clone containing the entire coding region of this snRNP core protein. Comparison of the 5'' end of this cDNA with the sequences of two processed pseudogenes and primer extension data suggest that the cDNA is nearly full length. The longest open reading frame in this clone codes for a basic 92 amino acid protein which is in perfect agreement with amino acid sequence data obtained from purified E protein. The predicted sequence of this protein reveals no extensive similarity to other snRNP proteins, but contains regions of similarity to a eukaryotic ribosomal protein.This publication has 31 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Human actin genes are single copy for alpha-skeletal and alpha-cardiac actin but multicopy for beta- and gamma-cytoskeletal genes: 3' untranslated regions are isotype specific but are conserved in evolution.Molecular and Cellular Biology, 1983
- Evolutionary history of a multigene family: An expressed human β-tubulin gene and three processed pseudogenesCell, 1983
- The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitroCell, 1983
- Location and structure of the var1 gene on yeast mitochondrial DNA: Nucleotide sequence of the 40.0 alleleCell, 1982
- Autoantibodies to Nuclear Antigens (ANA): Their Immunobiology and MedicinePublished by Elsevier ,1982
- Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosusProceedings of the National Academy of Sciences, 1979
- Prediction of the Secondary Structure of Proteins from their Amino Acid SequencePublished by Wiley ,1979
- The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNACell, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977