NEW EVIDENCE FOR THE PRESENCE OF INDOLEACETIC ACID IN TOBACCO

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Abstract
An acidic growth-promoting substance, active in the Avena coleoptile elongation test, was extracted from Resistant Havana 211 cultivar of Nicotiana tabacum L. This substance cochromatographed with indoleacetic acid (IAA) in three different solvent systems used singly, or in combination in two-direction chromatography. Its concentration–activity curve was also identical with that of IAA.Refinements in the purification procedure and the use of N,N-dimethylaminocinnamaldehyde as a spray reagent for the tobacco leaf extracts have made it possible to obtain a typical IAA color reaction at the Rf value of the tobacco growth-promoting substance. These results led to the conclusion that the auxin extracted from the tobacco tissues was IAA.