The liberation from yeast of substances giving the nitroprusside reaction

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Abstract
A large number of agents were examined as to their power to liberate from yeast substances giving the nitroprusside reaction. Positive results were obtained by a variety of methods, some physical (heating, freezing, grinding, ultra-violet light), some chemical (exposure to saturated salt solutions or to phenols and amines in solutions of 0.1-0.01 M). Negative results are more difficult to establish, because the nitroprusside test is inhibited by many compounds (NaNO2, ethyl alcohol, toluene); hence an apparent negative result with yeast may be in reality a positive one in which the color test is masked. The actors which produce from yeast substances giving the nitroprusside test can be separated to a certain extent into 2 classes: in one class, the liberation is chiefly in-tracellular; in the other it is extracellular also. Many of the positive agents are haemolytic (bile salts, saponin, sodium oleate, freezing) but lysis of the yeast cell was not observed in any instance.

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