SILVER STAIN OF UNCONCENTRATED CEREBRO SPINAL-FLUID - PRELIMINARY-RESULTS IN MULTIPLE-SCLEROSIS AND OTHER NEUROLOGICAL DISEASES

  • 1 January 1982
    • journal article
    • research article
    • Vol. 138  (4) , 317-325
Abstract
A new technique for the analysis of the CSF proteins was Ag staining after isoelectric focusing; immunofixation is possible. The technique is simple and results are obtained 6 h after sample deposition. If immunofixation is used longer than 24 h washing is necessary. It is highly sensitive and bands containing 25 ng of proteins can be clearly distinguished so that small volumes (10 .mu.l at mean) of unconcentrated CSF can be used. With this technique abnormal distinct bands of IgG specificity are seen in the majority of CNS and peripheral nervous system inflammatory disorders. Distribution of these bands, particularly in the more cathodal zone, seems to be non-specific for diseases including multiple sclerosis (MS). The abnormal IgG bands in MS CSF seem to have their counterpart in the serum. These results need further verification. The usefulness of this technique for research purposes is stressed. The protein pattern obtained must be interpreted in the light of the concepts of genetic polymorphism, microheterogeneity and heterogeneous mode of antibody production.