Flow injection–fluorimetric method for the determination of ranitidine in pharmaceutical preparations using o-phthalaldehyde

Publisher Website
Google Scholar
Abstract
A flow injection–fluorimetric procedure for the determination of ranitidine is proposed. The assay is based on the reaction of the drug with sodium hypochlorite, to produce a primary amine, which reacts with o-phthalaldehyde and 2-mercaptoethanol to form highly fluorescent derivatives. The calibration graph, based on peak area, is linear in the range 20–500 ng ml–1 of ranitidine with an accuracy of 3.4%. The corresponding detection limit is 13 ng ml–1(3.7 pmol). The method was applied to the determination of ranitidine in pharmaceuticals. The recovery was quantitative and no interferences from excipients were observed.

This publication has 30 references indexed in Scilit: