A Physicochemical Study of the High-Sulfur Proteins from Oxidized Wool
- 1 August 1963
- journal article
- research article
- Published by SAGE Publications in Textile Research Journal
- Vol. 33 (8) , 639-649
- https://doi.org/10.1177/004051756303300806
Abstract
High-sulfur proteins (γ-keratose) have been preferentially extracted from performic acid-oxidized wool with pyridine-acetate buffer (pH = 6.0). It has been shown that the γ-keratose is heterogeneous both on a charge and a molecular size basis by free electrophoresis, chromatography on DEAE-cellulose and calcium phosphate, and gel filtration on Sephadex. Four sub-fractions of γ-keratose which gave single peaks on electrophoresis and ultracentrifugation have been isolated by column electrophoresis. Thorough characterization has shown that these sub-fractions have very different physical properties and amino-acid compositions.Keywords
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