BONE-MARROW SUPPRESSOR B-CELLS INVITRO

  • 1 January 1978
    • journal article
    • research article
    • Vol. 35  (6) , 997-1003
Abstract
Marrow cells from intact CBA and (CBA .times. C57B1)F1 mice added to a culture of syngeneic splenocytes at its initiation suppressed the generation of anti-SRBC [sheep erythrocyte] antibody-forming cells. Removal of cells bearing surface immunoglobulins [Ig] and/or MBLA led to a substantial reduction in the marrow suppressive activity. Marrow cells from mice pretreated with 5 doses of hydroxyurea did not suppress the immune response of syngeneic splenocytes to SRBC. While the total number of cells in the marrow of such animals declined 5-fold, the relative content of Ig-positive cells was increased. The proportion of blast cells dropped from 10.3 to 2.4%. No blast cells bearing surface Ig were observed. The proportion of cells incorporating [3H]-thymidine fell 40-fold. Suppression of the immune response to SRBC in vitro is mediated by immature precursors of the B [bone marrow derived] lymphocyte series which are present in the bone marrow of intact mice.

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