The Use of "Tagged" Derivatives in the Fluorimetric Assay of Vitamins

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Abstract
In a study of the Konig reaction, it was found that nicotinic acid, on reacting with CNBr and certain substituted aromatic amines, yields glutaconic dialdehyde derivatives which fluoresce with a greenish-yellow light in visible violet light of about 440 millimicrons wave-length. A procedure was described which is applicable to 0.1 to 6 micrograms in 5 cc of solution or extract. It is suggested that the principle of preparing fluorescing "tagged" derivatives be applied to other vitamins of the B-complex.