Characterization of the Proton-Translocating Cytochrome c Oxidase Activity in the Plasma Membrane of Intact Anacystis nidulans Spheroplasts

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Abstract
Intact spheroplasts of the cyanobacterium (blue-green alga) A. nidulans oxidized various exogenous c-type cytochromes with concomitant outward H+ translocation while exogenous ferricytochrome c was not reduced. The H+/e- stoichiometry was close to 1 with each of the cytochromes and did not depend on the actual rate of the oxidase reaction. Observed H+ ejections were abolished by the uncoupler carbonyl cyanide m-chlorophenylhydrazone. Cyanide, azide and CO inhibited cytochrome c oxidation and H+ extrusion in parallel while dicyclohexylcarbodiimide affected H+ translocation more strongly than cytochrome c oxidation. The cytoplasmic membrane of A. nidulans appears to contain a H+-translocating cytochrome c oxidase similar to the one described for mitochondria.

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