The nature of the deoxyribonucleosides involved in the binding of carcinogenic hydrocarbons to the DNA of mouse embryo cells
- 15 June 1975
- journal article
- Published by Wiley in International Journal of Cancer
- Vol. 15 (6) , 912-917
- https://doi.org/10.1002/ijc.2910150606
Abstract
The DNA of mouse embryo cells was specifically labelled in the purine moieties with (G3H)‐deoxyadenosine or in the cytosine moieties with (53H)‐deoxycytidine. These cells were then treated with 7‐methylbenz (a) anthracene (7MBA) or benzo (a)‐pyrene (B(a)P) and the DNA isolated, degraded and fractionated by LH20 Sephadex column chromatography. When the purines of the DNA were tritium‐labelled, radioactive hydrocarbondeoxyribonucleoside products were obtained. No such products were found with deoxycytidine pre‐labelled DNA. Contrary to an earlier suggestion, these results indicate that it is the purine moieties of DNA which react with the metabolically activated hydrocarbon derivative in vivo.Keywords
This publication has 7 references indexed in Scilit:
- Metabolic activation of benzo(a)pyrene proceeds by a diol-epoxideNature, 1974
- Metabolic conversion of benzo[a]pyrene by Syrian hamster liver microsomes and binding of metabolites to deoxyribonucleic acidJournal of Medicinal Chemistry, 1973
- Reaction of 7-bromomethylbenz[a]anthracene with nucleic acids, polynucleotides, and nucleosidesBiochemistry, 1971
- Metabolism and binding to cellular macromolecules of a series of hydrocarbons by mouse embryo cells in cultureInternational Journal of Cancer, 1969
- Reaction of some mutagenic and carcinogenic compounds with nucleic acidsJournal of Cellular and Comparative Physiology, 1964
- Evidence for the Binding of Polynuclear Aromatic Hydrocarbons to the Nucleic Acids of Mouse Skin : Relation between Carcinogenic Power of Hydrocarbons and their Binding to Deoxyribonucleic AcidNature, 1964