Spin-labeled hemoglobin.

Abstract
A preliminary study of the paramagnetic resonance spectrumof spin-labeled horse hemoglobin in solution is described. Evidence is strong that on oxygenation the B chains undergo a significant structural change near the reactive sulfhydryl gray (B 93) a change not yet resolved by X-ray diffraction. Features were found in the paramagnetic resonance spectra of nitroxide-maleimide spin-labeled hemoglobin that appear to be closely related to observations of Benesch Benesch on the inhibition of the Bohr effect by N-ethylmallimide.