Mouse/Human T‐Cell Hybrids Rosetting with Sheep Erythrocytes

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Abstract
Hybrid cells were recovered from selective culture medium after fusion of concanavalin-A-activated human lymphocytes with an AKR mouse thymoma (BW 5147). After 6 mo. of culture 27 of 40 clones still contained human chromosomes. Human chromosome 6 was present in 89% of these clones, the human X in 70%. Clones from 1 hybrid line contained several human chromosomes. In 12 of the clones carrying human chromosomes, the rosetting with sheep erythrocytes (SRBC) was 3 times as high as in the BW 5147 cell lines. All these clones carried the human X chromosome as well. In some of these clones (25%) chromosome 6 was the only human one present. In the 6 clones in which human chromosome 6 was completely missing, rosetting with SRBC was at the level of the BW line. Genes on human chromosome 6 are apparently responsible for rosetting with SRBC.