Decreased protein level of nicotinic receptor α7 subunit in the frontal cortex from schizophrenic brain

Publisher Website
Google Scholar
Abstract
THE α7 subunit of nicotinic receptor (nAChR) was investigated in post-mortem brain tissue from eight schizophrenics and eight age-matched controls by Western blot. Using anti-α7 antibodies a single band with a mol. wt of 42 kDa was detected in human post-mortem brain, which was smaller in size than the single band (52 kDa) detected in SH-SY5Y and PC12 cells. The smaller band specifically bound to [125I]α-bungarotoxin, confirming the specificity of the detection in the human brain samples. A significant decrease in the level of α7 subunit protein was observed with the same method in the frontal cortex of schizophrenics compared with controls, while no difference was found in the parietal cortex. These findings suggest that a deficit of nAChR α7 subunit in the frontal cortex might be involved in the pathophysiology of schizophrenia.