A TESTED METHOD OF GROWING STENTOR COERULEUS

30 August 1940

journal article
research article
Published by American Association for the Advancement of Science (AAAS) in Science

Vol. 92 (2383) , 206
https://doi.org/10.1126/science.92.2383.206

Abstract

Gallon jars are nearly filled with spring water, and to each is added 10 ml. of an extract of lettuce, prepd. by boiling 10 g. of fresh lettuce leaves in 100 ml. of distilled water until about 50 ml. have been evaporated. After 12-24 hrs. the water begins to appear cloudy due to bacteria. Then specimens of S. coeruleus and Chilomonas paramecium are put in the jars. Every few days, when the water shows signs of becoming clear, fresh lettuce extract is added. Whenever (usually 2 to 4 wks.) the stentors begin to multiply less rapidly, new cultures are started by transferring some stentors and chilomonads from the old cultures to jars of new medium. The jars are kept at room temp. in light of very low intensity or in darkness.

This publication has 0 references indexed in Scilit: