Rapid micromethod for flourometric determination of neutral lipids on thin-layer chromatograms
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 39 (5) , 479-483
- https://doi.org/10.3109/00365517909106134
Abstract
A rapid fluorometric procedure for in situ evaluation of neutral lipids on TLC is described. The lipids are rendered fluorescent by treatment with perchloric acid and intensity of emitted light is recorded by flying spot scanning. Detection limits (2.8 .times. SD) are 0.10, 0.11 and 0.17 nmol for triglycerides, cholesterol and cholesterol esters, respectively. Range of linearity is 0-3.4 nmol for triglycerides, 0-2.0 nmol for cholesterol and 0-4.0 nmol for cholesterol esters. When standard in triplicate and 2 samples of each unknown are applied to the chromatogram, standard error of the mean is 2.7% for triglycerides, 1.7% for cholesterol and 1.0% for cholesterol esters. Sensitivity of the method allows simultaneous determination of the 3 lipid classes in 5 .mu.l lipid extract representing 0.238 .mu.l human plasma.This publication has 14 references indexed in Scilit:
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