Use of an Internal Standard to Assay 6 β-Hydroxycortisol in Urine
- 1 September 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (10) , 2051-2057
- https://doi.org/10.1080/01483918408068856
Abstract
Currently 6 β-hydroxycortisol is assayed by radio-immunoassay or high performance liquid chromatography techniques. We have developed an HPLC method, utilizing gradient elution and an internal standard {a 4-pregnene-tetrol-3-one}. In this way, accuracy and sensitivity of the assay were greatly improved and allowed the application of this modified method for monitoring the time-course of hepatic microsomal enzyme activity.This publication has 7 references indexed in Scilit:
- Brief technical note A new method for the determination of 6-beta-OH-cortisol in human urineClinica Chimica Acta; International Journal of Clinical Chemistry, 1981
- A direct enzyme immunoassay of 6.BETA.-hydroxycortisol in human urine.CHEMICAL & PHARMACEUTICAL BULLETIN, 1981
- The Estimation of 6β-Hydroxycortisol in Urine — A Comparison of Two Methods: High Performance Liquid Chromatography and Radioimmunoassaycclm, 1981
- Quantitative determination by HPLC of urinary 6?-hydroxycortisol, an indicator of enzyme induction by rifampicin and antiepileptic drugsEuropean Journal of Clinical Pharmacology, 1979
- A direct radioimmunoassay for 6β-hydroxycortisol in human urineThe Journal of Steroid Biochemistry and Molecular Biology, 1978
- Radioimmunoassay of 6β-hydroxycortisol in human plasma and urineSteroids, 1977
- 6β-HYDROXYCORTISOL AND OTHER POLAR CORTICOSTEROIDS: MEASUREMENT AND SIGNIFICANCE IN HUMAN URINE*†Journal of Clinical Endocrinology & Metabolism, 1961