The acid phosphatases of rat liver

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Abstract
The pH-activity curves were determined for the acid phosphatase activity of rat-liver homogenates with [beta]-glycerophosphate, phenyl phosphate, p-nitrophenyl phosphate and adenosine 3[image]-phosphate as substrates. These curves suggested the presence in rat liver of 2 non-specific acid phosphatases, with the possibility of a 3d showing some specificity towards [beta]-glycerophosphate and adenosine-3[image]-phosphate. The effect of various substances on the phosphatase activity of rat-liver homogenates was studied over a wide pH range and the results confirmed the presence of at least 2 acid phosphatases. Further evidence for the existence of 2 acid phosphatases was obtained from fractiona-tion studies involving acetone precipitation in the presence of Zn2+ ions and electrophoresis in the Tiselius apparatus. The intracellular distribution in rat liver of acid phosphatase activity was studied by differential centrifugation. No difference in the location of the different activities was apparent.